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human cancer tissue microarrays  (Novus Biologicals)


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    Structured Review

    Novus Biologicals human cancer tissue microarrays
    (A) Subcutaneous tumor growth with overexpression of 4 Raf1 constructs: WT Raf1 (n = 14), PM Raf1 (n = 8), Mito Raf1 (n = 8), and Mito Raf1 Kinase Dead (K375A) (n = 8); * P < 0.05, **** P < 0.0001. ( B ) Western blot of phosphorylated ERK and total ERK protein in cells expressing empty vector or mitochondrial Raf1 K375A. ( C ) With quantitation; P = ns. ( D ) PLA of spontaneous tumor <t>microarrays</t> for 3 normal and 3 squamous cell carcinoma (SCC) samples, including one matched. Scale bar represents 50 µm. ( E ) Density plot of puncta count per cell for PLA across several patient samples: 6 normal skin samples, and 26 SCC samples. Mean puncta count per cell is 6.928 for normal skin and 14.266 for SCC; **** P < 0.0001.
    Human Cancer Tissue Microarrays, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cancer tissue microarrays/product/Novus Biologicals
    Average 94 stars, based on 7 article reviews
    human cancer tissue microarrays - by Bioz Stars, 2026-04
    94/100 stars

    Images

    1) Product Images from "Mitochondrial Raf1 Regulates Glutamine Catabolism"

    Article Title: Mitochondrial Raf1 Regulates Glutamine Catabolism

    Journal: bioRxiv

    doi: 10.1101/2024.03.08.581297

    (A) Subcutaneous tumor growth with overexpression of 4 Raf1 constructs: WT Raf1 (n = 14), PM Raf1 (n = 8), Mito Raf1 (n = 8), and Mito Raf1 Kinase Dead (K375A) (n = 8); * P < 0.05, **** P < 0.0001. ( B ) Western blot of phosphorylated ERK and total ERK protein in cells expressing empty vector or mitochondrial Raf1 K375A. ( C ) With quantitation; P = ns. ( D ) PLA of spontaneous tumor microarrays for 3 normal and 3 squamous cell carcinoma (SCC) samples, including one matched. Scale bar represents 50 µm. ( E ) Density plot of puncta count per cell for PLA across several patient samples: 6 normal skin samples, and 26 SCC samples. Mean puncta count per cell is 6.928 for normal skin and 14.266 for SCC; **** P < 0.0001.
    Figure Legend Snippet: (A) Subcutaneous tumor growth with overexpression of 4 Raf1 constructs: WT Raf1 (n = 14), PM Raf1 (n = 8), Mito Raf1 (n = 8), and Mito Raf1 Kinase Dead (K375A) (n = 8); * P < 0.05, **** P < 0.0001. ( B ) Western blot of phosphorylated ERK and total ERK protein in cells expressing empty vector or mitochondrial Raf1 K375A. ( C ) With quantitation; P = ns. ( D ) PLA of spontaneous tumor microarrays for 3 normal and 3 squamous cell carcinoma (SCC) samples, including one matched. Scale bar represents 50 µm. ( E ) Density plot of puncta count per cell for PLA across several patient samples: 6 normal skin samples, and 26 SCC samples. Mean puncta count per cell is 6.928 for normal skin and 14.266 for SCC; **** P < 0.0001.

    Techniques Used: Over Expression, Construct, Western Blot, Expressing, Plasmid Preparation, Quantitation Assay



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    (A) Subcutaneous tumor growth with overexpression of 4 Raf1 constructs: WT Raf1 (n = 14), PM Raf1 (n = 8), Mito Raf1 (n = 8), and Mito Raf1 Kinase Dead (K375A) (n = 8); * P < 0.05, **** P < 0.0001. ( B ) Western blot of phosphorylated ERK and total ERK protein in cells expressing empty vector or mitochondrial Raf1 K375A. ( C ) With quantitation; P = ns. ( D ) PLA of spontaneous tumor <t>microarrays</t> for 3 normal and 3 squamous cell carcinoma (SCC) samples, including one matched. Scale bar represents 50 µm. ( E ) Density plot of puncta count per cell for PLA across several patient samples: 6 normal skin samples, and 26 SCC samples. Mean puncta count per cell is 6.928 for normal skin and 14.266 for SCC; **** P < 0.0001.
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    Image Search Results


    (A) Subcutaneous tumor growth with overexpression of 4 Raf1 constructs: WT Raf1 (n = 14), PM Raf1 (n = 8), Mito Raf1 (n = 8), and Mito Raf1 Kinase Dead (K375A) (n = 8); * P < 0.05, **** P < 0.0001. ( B ) Western blot of phosphorylated ERK and total ERK protein in cells expressing empty vector or mitochondrial Raf1 K375A. ( C ) With quantitation; P = ns. ( D ) PLA of spontaneous tumor microarrays for 3 normal and 3 squamous cell carcinoma (SCC) samples, including one matched. Scale bar represents 50 µm. ( E ) Density plot of puncta count per cell for PLA across several patient samples: 6 normal skin samples, and 26 SCC samples. Mean puncta count per cell is 6.928 for normal skin and 14.266 for SCC; **** P < 0.0001.

    Journal: bioRxiv

    Article Title: Mitochondrial Raf1 Regulates Glutamine Catabolism

    doi: 10.1101/2024.03.08.581297

    Figure Lengend Snippet: (A) Subcutaneous tumor growth with overexpression of 4 Raf1 constructs: WT Raf1 (n = 14), PM Raf1 (n = 8), Mito Raf1 (n = 8), and Mito Raf1 Kinase Dead (K375A) (n = 8); * P < 0.05, **** P < 0.0001. ( B ) Western blot of phosphorylated ERK and total ERK protein in cells expressing empty vector or mitochondrial Raf1 K375A. ( C ) With quantitation; P = ns. ( D ) PLA of spontaneous tumor microarrays for 3 normal and 3 squamous cell carcinoma (SCC) samples, including one matched. Scale bar represents 50 µm. ( E ) Density plot of puncta count per cell for PLA across several patient samples: 6 normal skin samples, and 26 SCC samples. Mean puncta count per cell is 6.928 for normal skin and 14.266 for SCC; **** P < 0.0001.

    Article Snippet: Proximity ligation of human cancer tissue microarrays (Skin cancer: NBP2-30229, Multiple cancers: NBP2-30263; Novus Biologicals) was used to analyze Raf-GLS protein-protein interaction in diverse cancer contexts.

    Techniques: Over Expression, Construct, Western Blot, Expressing, Plasmid Preparation, Quantitation Assay